How to Create a Spectral Unmixing Matrix

Spectral unmixing separates overlapping fluorophore signals, and its accuracy depends heavily on the quality of the unmixing matrix. This video walks through how to build an unmixing matrix in OMIQ from single-stained controls, review and adjust the generated gates, and apply the matrix to your experimental files.

How to Create a Spectral Unmixing Matrix

Learn how to build a spectral unmixing matrix in OMIQ from single-stained controls, apply it to your files, and set up the workflow branches, cleanup gates, and scaling needed for downstream spectral analysis.

LENGTH 5 Minutes

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